Pathogen Targeted Sequencing
PTseq™ Pathogen Targeted Sequencing
PTseq™ is based on ultra-multiplex PCR and targeted next-generation sequencing (tNGS). This method specifically amplifies target genes and then sequences them on a high-throughput platform. By comparing results against databases and using bioinformatics algorithms, PTseq™ identifies suspected pathogenic microorganisms and their resistance and virulence genes, increases the diagnostic positive rate, helps physicians develop personalized anti-infective treatment regimens, reduces the risk of drug resistance, and supports precise diagnosis and management of infections.
Clinical Challenges Addressed by PTseq™
PTseq™ is intended for patients with respiratory tract infections and can rapidly identify 274 common clinical pathogenic microorganisms, drug resistance genes, and virulence genes. This early detection, comprehensive analysis, and adjunctive treatment support can be life-saving and reduce medical costs, especially for patients who are hardest to diagnose or immunocompromised patients with a broader range of potential pathogens.
Recommended Population
Patients with Respiratory Tract Infections
Including upper respiratory tract infections, lower respiratory tract infections, tuberculosis infections, chronic respiratory infections, and others.
Sample Types
Respiratory Samples
PTseq™ is suitable for various respiratory samples: BALF, sputum, swabs, and other respiratory fluid samples such as lung fluid and endotracheal aspirates.
Detection Coverage
Local PTseq™ Solution
The solution includes all instrument modules, reagents, analysis software, and technology transfer services, enabling the entire process of sample processing, sequencing, and reporting to be carried out locally.
Why Choose PTseq™
High Specificity
With no background interference, positive enrichment, and species-specific sequences, the target detection rate increases significantly.
High Sensitivity
No background interference + positive enrichment.
Broad Coverage
Covers more than 95% of respiratory infection pathogens.
No Laboratory Culture Required
Direct nucleic acid extraction; microbial culture is not required.
Fast & Convenient
Simultaneous detection of DNA and RNA pathogens for rapid results.
Cost-Effective
Much lower cost and higher clinical applicability than mNGS (metagenomic next-generation sequencing).
Comprehensive Anti-Contamination System
Prevention of Intra-Batch Contamination Risk
Dual barcode technology (each sample is tagged with a unique label in each PCR cycle) enables accurate identification and correction through bioinformatics, while tightly controlling the risk of cross-contamination within a batch. The bioinformatics process automatically identifies and filters contaminated sequences to reduce the false-positive rate.
Prevention of Inter-Batch Contamination
Thermosensitive UDG enzyme technology is used to eliminate residual PCR products at the outset and prevent inter-batch contamination.
Patented Primer Design System
Database
Based on BGI's clinical-grade microbial sequence database, PTDB, ensuring precise selection of pathogen-specific regions.
Primer Design Algorithm
An efficient primer design algorithm ensures primer sequences are highly conserved within a pathogen species and highly specific for distinguishing between species.
Multi-Target Primer Design
The multi-target primer design strategy ensures efficient amplification of pathogen targets and avoids off-target detection: the positive rate increases significantly and the limit of detection (LOD) decreases significantly.
List of 274 Detectable Pathogen Targets
Bacteria (72)34 Gram-positive · 38 Gram-negative
Gram-positive (34): Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus constellatus, Streptococcus dysgalactiae, Staphylococcus aureus, Mycobacterium tuberculosis complex, Mycobacterium avium complex (MAC), Mycobacterium kansasii, Mycobacteroides abscessus, Mycobacteroides chelonae, Mycolicibacterium fortuitum, Enterococcus faecalis, Enterococcus faecium, Corynebacterium diphtheriae, Corynebacterium striatum, Rhodococcus equi, Listeria monocytogenes, Erysipelothrix rhusiopathiae, Clostridium perfringens, Bacillus anthracis, Nocardia (19 species), Tropheryma whipplei.
Gram-negative (38): Yersinia pestis, Pseudomonas aeruginosa, Haemophilus influenzae, Legionella pneumophila, Legionella bozemanae, Legionella micdadei, Klebsiella pneumoniae, Klebsiella aerogenes, Klebsiella oxytoca, Acinetobacter baumannii, Escherichia coli, Enterobacter cloacae, Proteus mirabilis, Proteus vulgaris, Proteus penneri, Streptobacillus moniliformis, Bordetella (5 species), Burkholderia mallei, Burkholderia pseudomallei, Burkholderia cepacia, Stenotrophomonas maltophilia, Morganella morganii, Salmonella enterica, Pasteurella multocida, Neisseria meningitidis, Moraxella catarrhalis, Bacteroides fragilis, Serratia marcescens, Fluoribacter dumoffii, Brucella, Francisella tularensis, Citrobacter freundii, Elizabethkingia meningoseptica, Achromobacter xylosoxidans.
Viruses (95)33 DNA · 62 RNA
DNA viruses (33): Monkeypox virus, Human bocavirus 1–4, Human adenovirus (A–E, including HAdV 1, 2, 5, 6, 7, 11, 14, 21, 34, 35, 56), Human alphaherpesvirus 1 & 2, Varicella-zoster virus, Human betaherpesvirus 5, 6A, 6B, Epstein-Barr virus, BK polyomavirus, JC polyomavirus.
RNA viruses (62): Influenza A (H1N1, H3N2, H5N1, H5N6, H7N9), Influenza B, Influenza C, Respiratory syncytial virus A & B, Human coronavirus OC43/229E/HKU1/NL63, SARS-CoV-2 (2019-nCoV), MERS-CoV, parainfluenza viruses 1–4, Measles morbillivirus, Human metapneumovirus, Rhinovirus A/B/C, Enterovirus A–D (including EV-A71, EV-D68), Coxsackievirus A2–A19 & B1–B6, Echovirus E4–E33, Human poliovirus 1, Parechovirus A, Rubella virus, Mumps rubulavirus.
Fungi (50)Molds, yeasts & dimorphic fungi
Candida albicans, Candida orthopsilosis, Candida parapsilosis, Candida glabrata, Candida tropicalis, Pichia kudriavzevii, Cryptococcus neoformans & complex (gattii, deuterogattii, bacillisporus, tetragattii), Histoplasma capsulatum, Talaromyces marneffei, Aspergillus (niger, fumigatus, flavus, lentulus, nidulans, oryzae, terreus), Lichtheimia corymbifera & ramosa, Rhizopus oryzae, Rhizopus microsporus, Rhizomucor pusillus, Actinomucor elegans, Mucor (circinelloides, indicus, irregularis, racemosus), Pneumocystis jirovecii, Coccidioides immitis & posadasii, Magnusiomyces capitatus, Trichosporon asahii, Scedosporium apiospermum, Clavispora lusitaniae, Blastomyces (dermatitidis, gilchristii, parvus), Cunninghamella bertholletiae & elegans, Apophysomyces elegans, Emergomyces (orientalis, pasteurianus), Fusarium solani, Geotrichum candidum, Sporothrix brasiliensis & schenckii, Syncephalastrum racemosum.
Other Pathogens (15)Mycoplasma · Chlamydia · Rickettsia · etc.
Mycoplasma pneumoniae, Chlamydia pneumoniae, Chlamydia psittaci, Chlamydia trachomatis, Bartonella bacilliformis, Bartonella henselae, Bartonella quintana, Rickettsia rickettsii, Rickettsia prowazekii, Rickettsia typhi, Orientia tsutsugamushi, Coxiella burnetii, Ureaplasma parvum, Ureaplasma urealyticum, Paragonimus westermani.
Drug Resistance Genes (31)IMP, NDM, KPC, OXA, CTX-M, mecA, vanA/B, etc.
IMP, NDM, SPM, KPC, PER, VIM, SIM, DIM, OXA, ACT, GIM, CTX-M, GES, VEB, CMY, OKP, TLA, ACC, MOX, MIR, QnrA, QnrB, mecA, MCR, vanA, vanB, vanC, SHV, FOX, sul, 23S rRNA.
Virulence Genes (11)rmpA, iutA, cnf1, tox, MPN372, etc.
rmpA, fyuA, fepA, iroN, iutA, cnf1, sat, eta, etb, tox, MPN372.